📢 From nanochannels to single‑protein reads — this issue highlights recent advances in gate‑tunable ion transport, DNA‑origami SERS, thermoplasmonic switching, and ultrafast nanopore analytics.
MSCA · GA 101072818MoS₂/SiN nanochannel (≈10 nm high) switches surface charge with voltage — positive or negative ions pass selectively. Ion‑based logic for brain‑like computing.
Unfolded BSA (SDS) pulled through the same channel. Clear current drops — proteins stay longer, enabling single‑molecule readout.
📌 “a big step toward reading single proteins one by one.”
DNA‑origami cages with AuNPs create programmable hotspots. Alkyne reporters at ~2125 cm⁻¹ confirm reliable positioning. Inner‑modified cages show richest SERS complexity.
Massively parallel single‑molecule melting across 10 temperatures (26–58 °C) for 79 duplexes. koff varies 10³‑fold; kon is nearly invariant.
Non‑monotonic temperature response: rate increases then decreases — explained by toehold hybridisation vs. branch migration. Mismatches enhance thermal sensitivity.
Markov chain modelAlexa 647 + Atto 647N ratiometric readout. DNA‑origami PAINT with ~30 nm precision — spatially resolved temperature mapping.
super‑resolution🧪 Translocation complexity: review article establishes framework for velocity, signal strength, event frequency. Minor labels (even 60× streptavidin) change velocity <2 % — nanoscale surprises.
Plasmonic bullseye nanopores focus light; magnetic switching traps/releases nanoparticles on demand. Enables longer observation and reconfigurable capture.
Single‑molecule fluorophore‑quencher melting curves. Experimental Tm ~10 °C higher than nearest‑neighbour. DNA anchors in nanopores for reversible trapping and ratchet transport.
ThermoSPARXS kinetic rules + strand‑displacement thermodynamics + SERS‑active nanocages + nanoscale thermometry → thermally switchable hybrid nanopores for multiplexed single‑molecule detection. Future work: plasmonic heating, controlled translocation, and temperature‑gradient mapping around plasmonic heaters.